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All tubes used were coated with 0.5% tissue culture grade BSA (A9576, Sigma-Aldrich) diluted in DPBS in order to prevent non-specific cell activation by adhesion to the tube wall. Isolated neutrophils ...

CP0406V2) and intranasally challenged with 250 ng of carrier-free recombinant murine IL-33 (R&D Systems, Catalog No. 3626-ML-010/CF) in 40 μL of Dulbecco’s Phosphate Buffered Saline (DPBS; Fisher ...

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Cells were treated for 1 minute with 1 mM H 2 O 2 to initiate biotinylating reaction and washed three times with Quencher solution (10 mM sodium ascorbate, 5 mM Trolox, 10 mM sodium azide in DPBS).