Two microliter PBS were added as a negative control. Cell count and cell viability were assessed using 1:1 trypan blue staining (Sigma-Aldrich) and an automated cell counter (Bio-Rad TC-20).

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After lysing the red blood cells with Red Blood Cell Lysis Solution (MACS) and removing dead cells with Dead Cell Removal Kit (MACS), the cells were counted and assessed for viability using Trypan ...

The mouse homolog shares 82–85% sequence identity ... Staining with the mitochondrial membrane potential dye, JC-1, and the trypan blue assay are previously described (2). Statistical ...

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Cell viability was determined by trypan blue exclusion, and differential counts were evaluated by May–Grunwald and Giemsa staining. BAL cells were either cytocentrifuged on slides and 2% formaldehyde ...

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