(G) Representative immunofluorescence images of Ub staining in cells treated with MG132 or transfected with various sPINK1 constructs, highlighting differences in ubiquitin localization and ...

Figure 3. Schematic of the antagonistic effect of viruses on SERINC5. HIV Nef, murine leukemia virus (MLV) GlycoGag, and equine infectious anemia virus (EIAV) S2 promote SERINC5 degradation and ...

Ubiquitin monomer, bovine serum albumin (BSA), Tris ... Roche), benzamidine (1 mM), PMSF (0.25 mM), and MG132 (10 μM). Lysates were clarified by centrifugation and quantified by Bradford assay. JOSD1 ...

Slides were then mounted in Vectashield medium containing DAPI solution. For nucleolus structural studies, 2 week-old Fib2-YFP plants (WT and nuc1.2, treated or not with MG132), grown on MS medium, ...