Autofluorescence is the natural fluorescence of biological structures such as mitochondria and lysosomes, in contrast to fluorescence originating from artificially added fluorescent markers (fluorophores).

2023年3月30日 · Common Sources of Autofluorescence in Life Science Research. To properly accommodate for the presence of autofluorescence in research imaging experiments, consider the biological components that comprise your sample. Here are some frequently observed autofluorescence producers that researchers encounter in the lab: 1. Nicotinamide adenine ...

2024年10月2日 · The term autofluorescence refers to any background fluorescence that occurs in cells or tissues that have not been stained with a specific fluorescent marker. It’s caused primarily by metabolites and cellular structures that serve as natural fluorophores within the cell.

2021年10月22日 · Native fluorescence, or autofluorescence (AF), consists in the emission of light in the UV-visible, near-IR spectral range when biological substrates are excited with light at suitable wavelength. This is a well-known phenomenon, and the strict ...

The best ways to address the issue of autofluorescence in order of preference: 1. Avoid it - not always possible. 2. Try to filter it out during image acquisition - difficult due to the broad emission spectrum. 3. Chemically remove it - can also reduce “real” signal.

Autofluorescence is the term given to describe the natural fluorescence that occurs in cells. The common compounds that give rise to this fluorescence signal include cyclic ring compounds like NAD(P)H, Collagen, and Riboflavin, as well as aromatic amino acids including tyrosine, tryptophan, phenylalanine.

Autofluorescence is the tissue-endogenous fluorescence caused by several different fluorophores. These include collagen and elastin as components of the connective tissue, tryptophan as a component of most proteins, and nicotinamide adenine dinucleotide (NAD), a …

Autofluorescence is the emission of fluorescent light from ocular structures in the absence of sodium fluorescein. Conditions that cause autofluorescence are optic nerve head drusen and astrocytic hamartoma ( Fig. 1.32 ).

Autofluorescence (primary fluorescence) is the fluorescence of naturally occurring substances, such as chlorophyll, collagen and fluorite. Most plant and animal tissues show some autofluorescence when excited with ultraviolet light (e.g. light of wavelength around 365 nm).

Autofluorescence (AF) refers to the inherent fluorescence exhibited by cells due to the presence of certain cellular components and metabolites that fluoresce upon excitation by specific wavelengths of light. 1 Common endogenous fluorophores include cyclic compounds such as reduced pyridine nucleotides (NADH), oxidized flavin coenzymes (FMN and ...