This review describes the history of overexpression, the mechanisms that are responsible for overexpression phenotypes, tests that begin to distinguish between those mechanisms, the varied ways in which overexpression is used, the methods and reagents available in several organisms, and the relevance of overexpression to human disease.

2023年12月20日 · Overexpression of genes plays a critical role in the development and progression of cancer. Unlike gene amplification, which refers to the increased copies of a particular gene within a genome, overexpression refers to the increased production of a gene’s protein products.

Gene overexpression refers to the activation of genes in aging cells, particularly those associated with the degradation of the extracellular matrix and the production of cytokines. It can lead to tissue damage and is believed to be influenced by gene dysregulation.

Gene Overexpression. In higher fungi, gene overexpression is mainly achieved by increasing the transcriptional level via using either endogenous or heterogeneous promoters.

Gene overexpression applications encompass both homologous and heterologous expression strategies. While homologous overexpression aims to amplify the activity of a resident chromosomal gene through increasing gene dosage, heterologous strategies are commonly utilized to impart a foreign or non-native activity to the host strain.

Overexpression experiments are sometimes considered as qualitative experiments designed to identify novel proteins and study their function. However, in order to draw conclusions regarding protein overexpression through association analyses using ...

2012年3月1日 · Overexpression of a gene can regulate the activation or suppression of other genes or gene members through various molecular mechanisms (Veitia et al. 2008; Prelich 2012). The data underscore the...

2013年7月30日 · Much of what is known about mammalian cell regulation has been achieved with the aid of transiently transfected cells. However, overexpression can violate balanced gene dosage, affecting protein...

This article reviews the history, applications, methods, mechanisms, and interpretation of overexpression phenotypes, focusing on its application in genetic screens but also providing examples of the utility of targeted overexpression.

The term “overexpression” predominates in studies involving unicellular organisms, implying increased expression beyond the norm, without any knowledge of the normal expression levels or pattern.