The single cell gel electrophoresis assay (SCGE, also known as comet assay) is an uncomplicated and sensitive technique for the detection of DNA damage at the level of the individual eukaryotic cell. It was first developed by Östling & Johansson in 1984 and later modified by Singh et al. in 1988. [ 1 ]

We describe here recent technical develop-ments, theoretical aspects, limitations as well as advantages of the assay, and modifications to measure cellular antioxi-dant status and different types of DNA repair. We briefly describe the applications of this method in genotoxicity testing, human biomonitoring, and ecogenotoxicology.

2015年8月12日 · The alkaline comet assay (single cell gel electrophoresis) is the most widely used method for measuring DNA damage in eukaryotic cells (Neri et al., 2015). It detects strand breaks (SBs) and alkali-labile sites at frequencies from a few hundred to several thousand breaks per cell—a biologically useful range, extending from low endogenous ...

2023年1月1日 · The Comet Assay, also known as single cell gel electrophoresis (SCRE), helps scientists to determine whether there has been deoxyribonucleic acid (DNA) damage to a single cell from...

2014年8月24日 · In a biochemical approach, substrate nucleoids containing specific DNA lesions are incubated with cell extract; repair enzymes in the extract induce breaks at damage sites; and the breaks are measured with the comet assay. The nature of the substrate lesions defines the repair pathway to be studied.

The comet assay (single-cell gel electrophoresis) is a simple method for measuring deoxyribonucleic acid (DNA) strand breaks in eukaryotic cells. Cells embedded in agarose on a microscope slide are lysed with detergent and high salt to form nucleoids containing supercoiled loops of DNA linked to the nuclear matrix.

Comet assay plays a crucial role in investigating the DNA damage and repair in different types of tumour cells (includes tumour cell lines and tumour cells extracted from cancer patients).

2008年4月22日 · The Comet assay has been widely accepted as a simple, sensitive, and rapid tool for assessing DNA damage and repair in individual eukaryotic as well as some prokaryotic cells, and has increasingly found application in diverse fields ranging from genetic toxicology to human epidemiology.

2019年5月23日 · Comet assays are used to determine the extent of DNA damage in a cell. This information can provide a great deal of useful information regarding the diagnosis and progression...

2021年1月5日 · The comet assay is a versatile method for measuring DNA strand breaks in individual cells. It can also be applied to cells isolated from treated animals. In this review, we highlight advantages and limitations of this in vivo comet assay in a regulatory context.