A new human acute monocytic leukemia cell line SHI-1 with t (6;11) (q27;q23), p53 gene alterations and high tumorigenicity in nude mice.

SHI-1 is a new monocytic leukemia cell line with the t(6;11) translocation, p53 gene alterations, and high tumorigenicity in nude mice. It could be a valuable tool in the study of leukemogenesis.

We have established a new monocytic cell line with t (6;11), designated SHI-1, and herein describe its biological characteristics. DESIGN AND METHODS: Mononuclear cells isolated from the bone marrow of a patient with acute monocytic leukemia (AML-M5b) at relapse were inoculated and passaged by liquid culture.

2005年6月1日 · SHI-1 is a new monocytic leukemia cell line with the t(6;11) translocation, p53 gene alterations, and high tumorigenicity in nude mice. It could be a valuable tool in the study of...

Multiplex RT-PCR was performed according to the method described by Pallisgaard et al.10 to detect the MLL partial tandem duplication (PTD) in SHI-1 cells. It used 29 sorts of primers for translocations and chro-mosomal aberrations in acute leukemia including the …

SHI-1 is a novel acute monocytic leukemia-derived cell line carrying t(6;11)(q27;q23) and p53 gene alteration and having high tumorigenicity in nude mice. It provides a new useful tool for leukemia research.

Cell line name SHI-1 : Species Homo sapiens (human) Cell type acute myelocytic leukemia : Incubation medium 80-90% Iscove's MDM + 10-20% h.i. FBS : Morphology round cells growing singly in suspension : Tumour type - RNA-seq project -

2019年12月19日 · SHI-1 is a new monocytic leukaemia cell line with t (6;11) translocation, p53 gene alterations, and high tumorigenicity in nude mice. SHI-1 could be a valuable tool in the study of leukemogenesis (Chen et al. 2005; Chen et al. 2007).

2005年2月1日 · SHI-1 is a novel acute monocytic leukemia-derived cell line carrying t(6;11)(q27;q23) and p53 gene alteration and having high tumorigenicity in nude mice. It provides a new useful tool for leukemia research.

2018年10月22日 · The present study determined that downregulating LPXN expression by L2-siRNA in SHI-1 cells could inhibit the secretion of MMP-2 and MMP-9, activate JNK and p38 MAPK, and weaken the malignant proliferation and the …